{"id":19,"date":"2026-10-12T11:01:54","date_gmt":"2026-10-12T11:01:54","guid":{"rendered":"https:\/\/fgvhp.com\/vhp-knowledge\/preparing-room-for-vhp-decontamination\/"},"modified":"2026-10-13T03:42:58","modified_gmt":"2026-10-13T03:42:58","slug":"preparing-room-for-vhp-decontamination","status":"publish","type":"post","link":"https:\/\/fgvhp.com\/es\/vhp-knowledge\/preparing-room-for-vhp-decontamination\/","title":{"rendered":"Preparaci\u00f3n de una sala para la descontaminaci\u00f3n con VHP: sellado, aislamiento del HVAC y colocaci\u00f3n"},"content":{"rendered":"\n\n<section class=\"phero\"><div class=\"wrap\">\n  <div class=\"crumb\"><a href=\"\/\">Home<\/a> \/ <a href=\"\/vhp-knowledge\/\">VHP Knowledge<\/a> \/ <span>Cycle design<\/span><\/div>\n  <span class=\"mono eyebrow\">Cycle design<\/span>\n  <h1>Preparing a Room for VHP Decontamination: Sealing, HVAC Isolation and Placement<\/h1>\n  <p class=\"lead\">Most failed room decontaminations are not caused by the generator. They are caused by leaks, cold spots, blocked surfaces and indicators in the wrong places. This guide walks through how to prepare a room before a VHP cycle and what to check during and after it.<\/p>\n  <div class=\"ameta\"><span>Fenggu VHP engineering team<\/span><span>4 min read<\/span><span>Updated October 2026<\/span><\/div>\n<\/div><\/section>\n\n<section class=\"fgv-sec\" style=\"padding-top:48px\"><div class=\"wrap\"><div class=\"art\">\n  <div class=\"keyp\"><b>Key points<\/b><ul><li>Define the boundary first: which rooms, which ducts, and what stays outside.<\/li><li>A room that leaks cannot hold concentration \u2014 seal doors, penetrations, drains and HVAC openings, then check for leaks during the cycle.<\/li><li>Open, separate and raise everything inside; remove cellulose and liquids.<\/li><li>Place indicators in the worst-case locations, not a convenient grid, and monitor adjacent areas.<\/li><\/ul><\/div>\n  <a class=\"dlbox\" href=\"\/resources\/room-vhp-decontamination-checklist\/\"><img decoding=\"async\" src=\"\/wp-content\/uploads\/site\/preview-room-checklist.png\" alt=\"\" loading=\"lazy\"><span><b>Free download: Room VHP decontamination checklist (PDF)<\/b><p>The checks in this guide as a printable two-page form, with an indicator log and sign-off. <span class=\"go\">Get it \u2192<\/span><\/p><\/span><\/a>\n  <h2>1. Define the boundary<\/h2>\n<p>Before anything else, decide exactly what is being decontaminated. One room, or a suite? Does the air handling ductwork need treatment as well, or only the room surfaces? Which adjoining spaces will stay occupied?<\/p>\n<p>The answer determines the equipment. A <a href=\"\/applications\/cleanroom-room-decontamination\/\">mobile generator<\/a> treats the room with the HVAC isolated. If the ducts are part of the contamination concern, or several rooms must be treated together, an <a href=\"\/applications\/hvac-integrated-vhp\/\">HVAC-integrated system<\/a> that distributes vapour through the air handling plant is usually the better fit.<\/p>\n<p>Record the boundary on a drawing, with the room volumes. Volume, together with the materials inside, is the starting point for dose and cycle design.<\/p>\n\n<h2>2. Survey what is in the room<\/h2>\n<ul>\n  <li><strong>Remove<\/strong> paper, cardboard, cotton, wood and liquids \u2014 they absorb vapour and lengthen aeration. See the <a href=\"\/vhp-knowledge\/vhp-material-compatibility\/\">material compatibility guide<\/a>.<\/li>\n  <li><strong>Open<\/strong> cabinet doors, drawers, equipment covers and biosafety cabinet sashes that should be treated.<\/li>\n  <li><strong>Separate and raise<\/strong> items so that surfaces are not stacked or resting flat on benches and floors.<\/li>\n  <li><strong>Clean first.<\/strong> VHP is not a cleaning agent. Visible soil, residues and films shield microorganisms from the vapour.<\/li>\n  <li><strong>Note heat sources and cold surfaces<\/strong> \u2014 fridges, incubators, external walls, chilled pipes. Cold surfaces are where condensation forms first.<\/li>\n<\/ul>\n\n<h2>3. Seal the room<\/h2>\n<p>A room has to hold concentration for the whole exposure phase. Leaks lower the dose at the walls and doors \u2014 often exactly where the hardest locations are \u2014 and expose people in adjacent areas.<\/p>\n<ul>\n  <li><strong>Doors:<\/strong> check gaskets; tape the perimeter of doors that are not airtight. Airtight doors with inflatable or mechanical seals remove the need for taping.<\/li>\n  <li><strong>Penetrations:<\/strong> cable trays, pipe entries, sockets, ceiling light fittings, sprinkler heads and access hatches.<\/li>\n  <li><strong>Drains and sinks:<\/strong> fill traps or cap the outlets.<\/li>\n  <li><strong>Pass-throughs:<\/strong> close both doors and include the pass box in the boundary or seal it off.<\/li>\n  <li><strong>HVAC:<\/strong> with a mobile generator, close supply and return dampers or seal the grilles, so vapour is neither exhausted nor carried into other zones. Plan how the HVAC will be restored for aeration.<\/li>\n<\/ul>\n<p>Decide whether the room is held at neutral pressure or slightly negative to its surroundings during the cycle, and make the decision part of the procedure. Outward leakage is a safety issue, not only an efficacy issue.<\/p>\n\n<figure>\n<svg viewBox=\"0 0 760 400\" role=\"img\" aria-label=\"Plan view of a room prepared for VHP: sealed door and grilles, generator, mixing fans, sensors and indicator locations\" font-family=\"IBM Plex Sans, Arial, sans-serif\">\n  <rect x=\"40\" y=\"30\" width=\"520\" height=\"340\" fill=\"#F5F8FB\" stroke=\"#0E1A2B\" stroke-width=\"4\"\/>\n  <line x1=\"40\" y1=\"250\" x2=\"40\" y2=\"330\" stroke=\"#D62B2B\" stroke-width=\"7\"\/>\n  <text x=\"52\" y=\"296\" font-size=\"11\" fill=\"#D62B2B\">sealed door<\/text>\n  <rect x=\"200\" y=\"22\" width=\"70\" height=\"16\" fill=\"#fff\" stroke=\"#D62B2B\" stroke-width=\"2\"\/><text x=\"235\" y=\"16\" font-size=\"11\" text-anchor=\"middle\" fill=\"#D62B2B\">supply grille sealed<\/text>\n  <rect x=\"420\" y=\"362\" width=\"70\" height=\"16\" fill=\"#fff\" stroke=\"#D62B2B\" stroke-width=\"2\"\/><text x=\"455\" y=\"394\" font-size=\"11\" text-anchor=\"middle\" fill=\"#D62B2B\">return grille sealed<\/text>\n  <rect x=\"80\" y=\"60\" width=\"150\" height=\"50\" fill=\"#DCE3EC\" stroke=\"#5A6B82\"\/><text x=\"155\" y=\"90\" font-size=\"12\" text-anchor=\"middle\" fill=\"#2B3A4F\">bench (items raised)<\/text>\n  <rect x=\"440\" y=\"60\" width=\"90\" height=\"120\" fill=\"#DCE3EC\" stroke=\"#5A6B82\"\/><text x=\"485\" y=\"115\" font-size=\"12\" text-anchor=\"middle\" fill=\"#2B3A4F\">cabinet<\/text><text x=\"485\" y=\"131\" font-size=\"11\" text-anchor=\"middle\" fill=\"#2B3A4F\">doors open<\/text>\n  <rect x=\"80\" y=\"300\" width=\"110\" height=\"50\" fill=\"#DCE3EC\" stroke=\"#5A6B82\"\/><text x=\"135\" y=\"330\" font-size=\"12\" text-anchor=\"middle\" fill=\"#2B3A4F\">equipment<\/text>\n  <rect x=\"265\" y=\"180\" width=\"70\" height=\"50\" rx=\"6\" fill=\"#003AA1\"\/><text x=\"300\" y=\"210\" font-size=\"12\" text-anchor=\"middle\" fill=\"#fff\" font-weight=\"600\">VHP<\/text>\n  <g fill=\"none\" stroke=\"#0A8F8A\" stroke-width=\"2\"><circle cx=\"150\" cy=\"200\" r=\"16\"\/><circle cx=\"430\" cy=\"290\" r=\"16\"\/><\/g>\n  <g stroke=\"#0A8F8A\" stroke-width=\"2\"><line x1=\"140\" y1=\"200\" x2=\"160\" y2=\"200\"\/><line x1=\"150\" y1=\"190\" x2=\"150\" y2=\"210\"\/><line x1=\"420\" y1=\"290\" x2=\"440\" y2=\"290\"\/><line x1=\"430\" y1=\"280\" x2=\"430\" y2=\"300\"\/><\/g>\n  <g fill=\"#D62B2B\"><rect x=\"52\" y=\"42\" width=\"10\" height=\"10\"\/><rect x=\"538\" y=\"42\" width=\"10\" height=\"10\"\/><rect x=\"52\" y=\"348\" width=\"10\" height=\"10\"\/><rect x=\"538\" y=\"348\" width=\"10\" height=\"10\"\/><rect x=\"480\" y=\"150\" width=\"10\" height=\"10\"\/><rect x=\"200\" y=\"335\" width=\"10\" height=\"10\"\/><rect x=\"150\" y=\"118\" width=\"10\" height=\"10\"\/><rect x=\"295\" y=\"100\" width=\"10\" height=\"10\"\/><rect x=\"66\" y=\"270\" width=\"10\" height=\"10\"\/><\/g>\n  <g fill=\"#FFB020\" stroke=\"#7A5200\"><polygon points=\"300,120 308,134 292,134\"\/><polygon points=\"520,250 528,264 512,264\"\/><polygon points=\"120,240 128,254 112,254\"\/><\/g>\n  <g font-size=\"13\" fill=\"#2B3A4F\">\n    <rect x=\"585\" y=\"40\" width=\"160\" height=\"230\" rx=\"10\" fill=\"#fff\" stroke=\"#DCE3EC\"\/>\n    <rect x=\"602\" y=\"64\" width=\"22\" height=\"16\" rx=\"3\" fill=\"#003AA1\"\/><text x=\"634\" y=\"77\">Generator<\/text>\n    <circle cx=\"613\" cy=\"106\" r=\"10\" fill=\"none\" stroke=\"#0A8F8A\" stroke-width=\"2\"\/><text x=\"634\" y=\"111\">Mixing fan<\/text>\n    <polygon points=\"613,132 621,146 605,146\" fill=\"#FFB020\" stroke=\"#7A5200\"\/><text x=\"634\" y=\"145\">H\u2082O\u2082 \/ RH \/ T<\/text><text x=\"634\" y=\"161\">sensor<\/text>\n    <rect x=\"608\" y=\"180\" width=\"10\" height=\"10\" fill=\"#D62B2B\"\/><text x=\"634\" y=\"190\">BI \/ CI<\/text><text x=\"634\" y=\"206\">(worst case)<\/text>\n    <line x1=\"602\" y1=\"236\" x2=\"624\" y2=\"236\" stroke=\"#D62B2B\" stroke-width=\"5\"\/><text x=\"634\" y=\"241\">Sealed<\/text>\n  <\/g>\n  <text x=\"300\" y=\"250\" font-size=\"11\" text-anchor=\"middle\" fill=\"#5A6B82\">jet not aimed at surfaces<\/text>\n<\/svg>\n<figcaption>Illustrative plan. Indicators sit in corners, behind and inside equipment, near the door and under fixtures \u2014 the places vapour reaches last, not a convenient grid.<\/figcaption>\n<\/figure>\n\n<h2>4. Place the generator and fans<\/h2>\n<ul>\n  <li>Put a mobile generator where its output can mix with the whole room, usually near the centre, and never aimed directly at a nearby surface \u2014 a concentrated jet on a cold surface is a condensation point.<\/li>\n  <li>Use mixing fans to move vapour into corners, behind equipment and into alcoves. Fan positions are part of the cycle and should be fixed in the procedure once validated.<\/li>\n  <li>Keep the generator&#8217;s own sensors and cables clear of doors and seals.<\/li>\n<\/ul>\n\n<h2>5. Sensors and indicators<\/h2>\n<p>Hydrogen peroxide concentration, relative humidity and temperature should be monitored at representative points, including at least one location expected to be worst case. Biological indicators (BIs) and chemical indicators (CIs) go where the vapour is least likely to reach the required dose:<\/p>\n<ul>\n  <li>Corners at floor and ceiling level, and the corners furthest from the generator.<\/li>\n  <li>Behind and underneath fixed equipment, inside cabinets and equipment housings.<\/li>\n  <li>Near the door and near any sealed opening where leakage would dilute the vapour.<\/li>\n  <li>Next to absorbent materials that could not be removed, and on cold surfaces.<\/li>\n<\/ul>\n<p>The number and positions come from the mapping study for the room. Once validated, the same positions are used for routine monitoring and periodic requalification, so record them on the room drawing.<\/p>\n\n<h2>6. Safety before, during and after<\/h2>\n<ul>\n  <li>Post warning signs on every entrance and control access to the room and the plant room serving it.<\/li>\n  <li>Monitor adjacent areas and door seals for leakage during exposure, using a low-range detector.<\/li>\n  <li>Define who can stop the cycle and how, and what happens if a leak is detected.<\/li>\n  <li>Release the room only after a documented reading at or below the limit \u2014 see <a href=\"\/vhp-knowledge\/vhp-aeration-residual-h2o2\/\">aeration and residual limits<\/a>.<\/li>\n<\/ul>\n\n<h2>Checklist<\/h2>\n<div class=\"tbl\"><table>\n<thead><tr><th>When<\/th><th>Check<\/th><\/tr><\/thead>\n<tbody>\n<tr><th>Planning<\/th><td>Boundary drawing and volumes \u00b7 material list \u00b7 equipment type confirmed \u00b7 BI\/CI positions defined \u00b7 adjacent areas identified<\/td><\/tr>\n<tr><th>Day before<\/th><td>Room cleaned \u00b7 cellulose and liquids removed \u00b7 drains capped \u00b7 cabinets and drawers open \u00b7 items raised and separated<\/td><\/tr>\n<tr><th>Before start<\/th><td>Doors and penetrations sealed \u00b7 HVAC dampers closed or grilles sealed \u00b7 generator and fans in validated positions \u00b7 indicators placed and logged \u00b7 signs posted \u00b7 room empty<\/td><\/tr>\n<tr><th>During<\/th><td>Concentration, RH and temperature trending as expected \u00b7 adjacent areas and door seals checked for leakage \u00b7 no condensation alarms<\/td><\/tr>\n<tr><th>After<\/th><td>Aeration to limit \u00b7 release reading recorded \u00b7 seals and tape removed \u00b7 HVAC restored \u00b7 indicators retrieved and sent for incubation \u00b7 cycle record signed<\/td><\/tr>\n<\/tbody><\/table><\/div>\n  <figure class=\"photo\"><img decoding=\"async\" src=\"\/wp-content\/uploads\/site\/cutout-vhpm500.jpg\" alt=\"Fenggu FG-VHP-M500 mobile VHP room sterilizer\" loading=\"lazy\" style=\"max-height:380px;object-fit:contain;padding:20px 0;background:#fff\"><figcaption>Fenggu FG-VHP-M500 mobile VHP room sterilizer, used for rooms up to 300 m\u00b3.<\/figcaption><\/figure>\n  <div class=\"refs\"><p><b>References<\/b><\/p><p>ISO 22441:2022 \u2014 Low temperature vaporized hydrogen peroxide sterilization<\/p><p>EU GMP Annex 1 (2022), Manufacture of Sterile Medicinal Products<\/p><p>PDA Technical Report No. 51, Biological Indicators for Gas and Vapor-Phase Decontamination Processes<\/p><\/div>\n<\/div><\/div><\/section>\n\n<section class=\"fgv-sec alt\"><div class=\"wrap\">\n  <span class=\"mono eyebrow\">Equipment<\/span><h2>Fenggu equipment for this<\/h2>\n  <div class=\"opts\"><a class=\"opt\" href=\"https:\/\/fenggu-bio.com\/products\/mobile-vhp-room-sterilizer\/\" target=\"_blank\" rel=\"noopener\"><span class=\"mono\">FG-VHP-M500<\/span><h3>Mobile VHP room sterilizer<\/h3><p>Wheeled unit for spaces up to 300 m\u00b3, with scheduled and remote start.<\/p><span class=\"go\">View specifications \u2192<\/span><\/a><a class=\"opt\" href=\"https:\/\/fenggu-bio.com\/products\/hvac-integrated-vhp-system\/\" target=\"_blank\" rel=\"noopener\"><span class=\"mono\">FG-VHP-C1000 \/ C2000<\/span><h3>HVAC-integrated VHP system<\/h3><p>For suites and ductwork: a fixed system that distributes vapour through the existing AHU.<\/p><span class=\"go\">View specifications \u2192<\/span><\/a><a class=\"opt\" href=\"https:\/\/fenggu-bio.com\/products\/inflatable-seal-airtight-door\/\" target=\"_blank\" rel=\"noopener\"><span class=\"mono\">Airtight doors<\/span><h3>Inflatable-seal airtight door<\/h3><p>Removes the need to tape doors on rooms that are decontaminated routinely.<\/p><span class=\"go\">View specifications \u2192<\/span><\/a><\/div>\n  <p style=\"margin-top:24px\">Related application guides: <a class=\"go\" href=\"\/applications\/cleanroom-room-decontamination\/\">Cleanroom and room decontamination<\/a> \u00b7 <a class=\"go\" href=\"\/applications\/biosafety-lab-decontamination\/\">Biosafety laboratory decontamination<\/a><\/p>\n<\/div><\/section>\n\n<section class=\"fgv-sec\"><div class=\"wrap narrow\">\n  <span class=\"mono eyebrow\">FAQ<\/span><h2>Common questions<\/h2>\n  <div class=\"faq\"><details><summary>Do I need to switch off and seal the HVAC for room VHP decontamination?<\/summary><p>With a mobile generator, yes: close the supply and return dampers or seal the grilles so vapour is not exhausted or carried into other zones, then restore the HVAC for aeration. With an HVAC-integrated system the air handling plant is used to distribute the vapour, so the approach is different.<\/p><\/details><details><summary>What should be removed from a room before VHP?<\/summary><p>Paper, cardboard, cotton, wood and liquids, because they absorb hydrogen peroxide and lengthen aeration. Everything else that needs treatment should be cleaned, opened and separated so the vapour can reach its surfaces.<\/p><\/details><details><summary>How many biological indicators are needed for a room?<\/summary><p>The number and positions come from the mapping study for that room and are justified by its size, layout and worst-case locations. Once validated, the same positions are used for routine monitoring and requalification.<\/p><\/details><details><summary>Where should biological indicators be placed?<\/summary><p>In the locations the vapour is least likely to reach at full dose: far corners at floor and ceiling level, behind and under fixed equipment, inside cabinets, near doors and seals, near absorbent materials and on cold surfaces.<\/p><\/details><details><summary>Should mixing fans be used with a mobile VHP generator?<\/summary><p>In most rooms, yes. Fans help vapour reach corners and spaces behind equipment and reduce the risk of local condensation. Their positions should be fixed during cycle development and kept the same for routine cycles.<\/p><\/details><\/div>\n<\/div><\/section>\n<script type=\"application\/ld+json\">{\"@context\":\"https:\/\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"Do I need to switch off and seal the HVAC for room VHP decontamination?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"With a mobile generator, yes: close the supply and return dampers or seal the grilles so vapour is not exhausted or carried into other zones, then restore the HVAC for aeration. With an HVAC-integrated system the air handling plant is used to distribute the vapour, so the approach is different.\"}},{\"@type\":\"Question\",\"name\":\"What should be removed from a room before VHP?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Paper, cardboard, cotton, wood and liquids, because they absorb hydrogen peroxide and lengthen aeration. 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Their positions should be fixed during cycle development and kept the same for routine cycles.\"}}]}<\/script>\n\n<section class=\"fgv-sec\" style=\"padding-top:0\"><div class=\"wrap\"><div class=\"cta\">\n  <div><span class=\"mono eyebrow\">Free application review<\/span><h2>Have a specific space or load in mind?<\/h2><p>Send the volume, the materials and what you need to prove. An engineer replies with a recommended setup and what validation will involve.<\/p><\/div>\n  <div><div class='fluentform ff-default fluentform_wrapper_1 ffs_default_wrap'><form data-form_id=\"1\" id=\"fluentform_1\" class=\"frm-fluent-form fluent_form_1 ff-el-form-top ff_form_instance_1_1 ff-form-loading ffs_default\" data-form_instance=\"ff_form_instance_1_1\" method=\"POST\" data-turnstile_key=\"0x4AAAAAAFR_A6IRFH-zWvr4\" ><fieldset  style=\"border: none!important;margin: 0!important;padding: 0!important;background-color: transparent!important;box-shadow: none!important;outline: none!important; min-inline-size: 100%;\">\n                    <legend class=\"ff_screen_reader_title\" style=\"display: block; margin: 0!important;padding: 0!important;height: 0!important;text-indent: -999999px;width: 0!important;overflow:hidden;\">Contact Form Demo<\/legend>        <div\n                style=\"display: none!important; position: absolute!important; transform: 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